Program
Provisional Timetable
Course hours – UK time
14:00 – 17:00
Subject to permission from the speakers, lectures will be recorded and made available to registered participants for the duration of the course and for a short time after it has concluded. This will allow you to catch up on any lectures you are unable to attend and also provide a backup in case of connection problems.
WEDNESDAY – September 1st
Introduction
David Ogden
Channel evolution and electrical excitability: Research at the MBA
Colin Brownlee
The squid and its giant nerve fibre – Squid axon voltage clamp
Film
THURSDAY – September 2nd
Voltage clamp methods: Two microelectrode clamp – Single electrode ‘switch’ clamp
Vincenzo Marra, Sandy Harper, Alasdair Gibb, Jon Robbins
FRIDAY – September 3rd
Patch clamp methods: High resolution single channel recording – Whole cell recording – Permeabilised patch recording
David Ogden, Boris Barbour, Marco Beato, Stephanie Schorge, Karri Lamsa
MONDAY – September 6th
Data analysis and interpretation: Single channel analysis – Whole cell clamp analysis – Interpretation and Statistics
Lucia Sivilotti, David Colquhoun, John Dempster
TUESDAY – September 7th
Bilayer recording and intracellular channels
Samantha Pitt
Dynamic Conductance Clamp
Stephen Brickley
WEDNESDAY – September 8th
Ion selective Microelectrodes: Making ISMEs, Electrochemistry of solution
Lydie Vargova, Juha Voipio
THURSDAY – September 9th
Extracellular recording
Clement Lena
Multielectrode arrays
FRIDAY – September 10th
Microscope optics, Fluorescence microscopy and Cameras
Brad Amos, Noah Russell, Jeremy Graham
MONDAY – September 13th
Fluorescent Ca indicators. Organic and GECI indicators
Martin Thomas, Katalin Torok
TUESDAY – September 14th
Optical imaging of membrane potential
Marco Canepari
Photolysis
David Ogden
WEDNESDAY – September 15th
Virus transfection methods
Jon Bradley
Optogenetic stimulation and inhibition
James Dillon
THURSDAY – September 16th
Electrical and optical recording of secretion and Endocytosis
Liz Seward, Tom Carter
FRIDAY – September 17th
Single cell cardiovascular physiology
Rich Rainbow
Whole cell recording in vivo and neuronal tracing